| Field | Specification |
|---|---|
| Target | |
| Alternative names | Probable ATP-dependent RNA helicase DDX6; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK; DDX6; HLR2; RCK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DDX6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18D64 is a rabbit monoclonal antibody that recognizes probable ATP-dependent RNA helicase DDX6 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 54.4 kDa, and the supplier reports an observed band at about 54 kDa.
DDX6 (also called RCK or p54) is a DEAD-box RNA helicase that is essential for forming P-bodies, cytoplasmic granules where translationally silent mRNAs are stored or degraded. It links translational repression with mRNA decapping and supports microRNA-mediated silencing. DDX6 is studied in mRNA storage, decay and stress responses.
| Target | Probable ATP-dependent RNA helicase DDX6 (gene DDX6; UniProt P26196, human) |
|---|---|
| Clone | 18D64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.4 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DDX6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places probable ATP-dependent RNA helicase DDX6 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Abundantly expressed in most tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
