| Field | Specification |
|---|---|
| Target | |
| Alternative names | Decorin;Bone proteoglycan II;PG-S2;PG40;DCN;SLRR1B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Decorin recombinant protein (Position: D31-K359). Human Decorin shares 80% and 77% amino acid (aa) sequences identity with mouse and rat Decorin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets decorin (gene DCN) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 39.7 kDa, and the supplier reports an observed band at about 70 kDa.
Decorin (DCN) is a small leucine-rich proteoglycan of the extracellular matrix that binds type I collagen and regulates the rate and organization of collagen fibril assembly. It can also bind TGF-β and several receptor tyrosine kinases and damp their signaling. Decorin is studied in connective tissue biology, fibrosis, wound healing and tumor growth.
| Target | Decorin (gene DCN; UniProt P07585, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 39.7 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells, human HepG2 cells, human SW620 cells, rat liver tissue, rat RH35 cells, mouse HEPA1–6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Decorin fragment (Asp31–Lys359), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 80% and 77%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes decorin as a secreted protein. Tissue expression noted by UniProt (human): Detected in placenta (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lai Jinsheng et al. (2017) Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling. Scientific Reports. 10.1038/srep44473
Kai Li et al. (2025) High‐Fat and High‐Sugar Diet Induces Murine Premature Ovarian Failure by Promoting Mitochondrial Oxidative Phosphorylation Response. Food Science & Nutrition. 10.1002/fsn3.70973
Xue Hui et al. (2019) Glycosylation of dentin matrix protein 1 is critical for fracture healing via promoting chondrogenesis. Frontiers of Medicine. 10.1007/s11684-019-0693-9
