| Field | Specification |
|---|---|
| Target | |
| Alternative names | MAP kinase-activating death domain protein; Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; Rab3 GDP/GTP exchange factor; RabGEF; Rab3 GDP/GTP exchange protein; Rab3GEP; MADD; DENN; IG20; KIAA0358 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DENN |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28M86 is a rabbit monoclonal antibody that recognizes MAP kinase-activating death domain protein (gene MADD) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 183.3 kDa, and the supplier reports an observed band at about 230 kDa.
MADD (MAP kinase-activating death domain protein, also known as DENN/MADD or Rab3GEP) is a guanine nucleotide exchange factor for Rab GTPases, including RAB3 and RAB27 family members. By activating these Rabs it supports synaptic vesicle release and regulated secretion, and it has also been linked to TNF receptor signaling and cell survival.
| Target | MAP kinase-activating death domain protein (gene MADD; UniProt Q8WXG6, human) |
|---|---|
| Clone | 28M86 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 183.3 kDa |
| Observed MW | 230 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DENN.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places MAP kinase-activating death domain protein in the cell membrane, cytoplasm and cell projection. Tissue expression noted by UniProt (human): Expressed in testis, ovary, brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
