| Field | Specification |
|---|---|
| Target | |
| Alternative names | Desmin; DES |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequence identity with both mouse and rat Desmin. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 2B5 is a mouse monoclonal antibody that recognizes desmin in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 53.5 kDa, and the supplier reports an observed band at about 54 kDa.
Desmin (DES) is the main intermediate filament protein of skeletal, cardiac and smooth muscle. It links neighboring myofibrils at the Z-disks and connects them to the sarcolemma, nucleus and mitochondria, helping muscle cells resist mechanical stress. Desmin is a standard muscle marker, and mutations cause desmin-related myopathies and cardiomyopathies.
| Target | Desmin (gene DES; UniProt P17661, human) |
|---|---|
| Clone | 2B5 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 53.5 kDa |
| Observed MW | 54 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue, human K562 cells, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human oesophagus squama cancer tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human Desmin fragment (Met1–Thr304), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places desmin in the cytoplasm, cell membrane, nucleus and cell tip. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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