| Field | Specification |
|---|---|
| Target | |
| Alternative names | Desmoglein-3; 130 kDa pemphigus vulgaris antigen; PVA; Cadherin family member 6; DSG3; CDHF6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Desmoglein 3/PVA/DSG3 recombinant protein (Position: R384-Q957). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes desmoglein-3 (gene DSG3) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 107.5 kDa, and the supplier reports an observed band at about 130 kDa.
Desmoglein-3 (DSG3) is a desmosomal cadherin found in the basal layers of the epidermis and oral mucosa. It is needed for desmosome assembly and firm keratinocyte adhesion, and it is the main autoantigen in pemphigus vulgaris.
| Target | Desmoglein-3 (gene DSG3; UniProt P32926, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 107.5 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human Hacat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human Desmoglein 3/PVA/DSG3 fragment (Arg384–Gln957), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places desmoglein-3 in the cell membrane, cell junction and cytoplasm. Tissue expression noted by UniProt (human): Expressed throughout the basal and spinous layer of the epidermis with weak expression in the granular layer (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
