| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dihydrofolate reductase; DHFR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 3C8 is a mouse monoclonal antibody that recognizes dihydrofolate reductase (gene DHFR) in human and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 21.5 kDa, and the supplier reports an observed band at about 22 kDa.
Dihydrofolate reductase (DHFR) uses NADPH to reduce dihydrofolate to tetrahydrofolate, regenerating the folate cofactors needed to make thymidylate, purines and glycine. This makes it essential for DNA synthesis and cell proliferation. DHFR is the target of methotrexate and is studied in folate metabolism and antifolate drug research.
| Target | Dihydrofolate reductase (gene DHFR; UniProt P00374, human) |
|---|---|
| Clone | 3C8 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 21.5 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human SGC-7901 cells, human HeLa cells, human SW620 cells, rat kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human rectal cancer tissue, rat gaster tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human DHFR fragment (Val2–Asp187), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places dihydrofolate reductase in the mitochondrion, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tian Linli et al. (2013) MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma. Medical Oncology. 10.1007/s12032-013-0785-3
