| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dihydrofolate reductase; DHFR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DHFR Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACBE-4 is a rabbit monoclonal antibody that recognizes dihydrofolate reductase (gene DHFR) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 21.5 kDa, and the supplier reports an observed band at about 21 kDa.
Dihydrofolate reductase (DHFR) uses NADPH to reduce dihydrofolate to tetrahydrofolate, regenerating the folate cofactors needed to make thymidylate, purines and glycine. This makes it essential for DNA synthesis and cell proliferation. DHFR is the target of methotrexate and is studied in folate metabolism and antifolate drug research.
| Target | Dihydrofolate reductase (gene DHFR; UniProt P00374, human) |
|---|---|
| Clone | ACBE-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.5 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human DHFR Key enzyme in folate metabolism.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dihydrofolate reductase in the mitochondrion, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
