| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein diaphanous homolog 1; Diaphanous-related formin-1; DRF1; DIAPH1; DIAP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DIAPH1 recombinant protein (Position: D1039-Q1153). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein diaphanous homolog 1 (gene DIAPH1) in human and monkey samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 141.3 kDa, and the supplier reports an observed band at about 150 kDa.
DIAPH1 (mDia1) is a formin that nucleates and elongates unbranched actin filaments, building structures such as stress fibers. It is activated by RhoA and also influences microtubule stability and SRF-dependent transcription. DIAPH1 is studied in cytoskeletal organization, cell migration, cytokinesis and hearing loss.
| Target | Protein diaphanous homolog 1 (gene DIAPH1; UniProt O60610, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 141.3 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human Raji cells, human HeLa cells, monkey COS-7 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human DIAPH1 fragment (Asp1039–Gln1153), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and monkey. UniProt places protein diaphanous homolog 1 in the cell membrane, cell projection, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
