| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein diaphanous homolog 1; Diaphanous-related formin-1; DRF1; DIAPH1; DIAP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DIAPH1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20D39 is a rabbit monoclonal antibody that recognizes protein diaphanous homolog 1 (gene DIAPH1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 141.3 kDa, and the supplier reports an observed band at about 150 kDa.
DIAPH1 (mDia1) is a formin that nucleates and elongates unbranched actin filaments, building structures such as stress fibers. It is activated by RhoA and also influences microtubule stability and SRF-dependent transcription. DIAPH1 is studied in cytoskeletal organization, cell migration, cytokinesis and hearing loss.
| Target | Protein diaphanous homolog 1 (gene DIAPH1; UniProt O60610, human) |
|---|---|
| Clone | 20D39 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 141.3 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human A549 cells, human HeLa cells, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human DIAPH1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein diaphanous homolog 1 in the cell membrane, cell projection, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
