| Field | Specification |
|---|---|
| Target | |
| Alternative names | Disrupted in schizophrenia 1 protein; DISC1; KIAA0457 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DISC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22D79 is a rabbit monoclonal antibody that recognizes disrupted in schizophrenia 1 protein (gene DISC1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 93.6 kDa, and the supplier reports an observed band at about 100 kDa.
DISC1 (disrupted in schizophrenia 1) is a scaffold protein that interacts with many partners at the centrosome, microtubules and synapses. It influences neural progenitor proliferation, neuronal migration and synapse development, including through Wnt/GSK3β signaling. DISC1 was identified at a chromosomal translocation in a family with major mental illness and is studied in neurodevelopment.
| Target | Disrupted in schizophrenia 1 protein (gene DISC1; UniProt Q9NRI5, human) |
|---|---|
| Clone | 22D79 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 93.6 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells, RAW264.7 cells, PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DISC1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places disrupted in schizophrenia 1 protein in the cytoplasm, mitochondrion and postsynaptic density. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
