| Field | Specification |
|---|---|
| Target | |
| Alternative names | Doublesex- and mab-3-related transcription factor 1;DM domain expressed in testis protein 1;DMRT1;DMT1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human DMRT1, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes doublesex- and mab-3-related transcription factor 1 (gene DMRT1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 39.5 kDa, and the supplier reports an observed band at about 39 kDa.
DMRT1 is a transcription factor with a DM DNA-binding domain that is central to male sex determination and testis development. It maintains Sertoli cell identity and controls the switch between mitosis and meiosis in spermatogonia. DMRT1 is conserved across vertebrates and is studied in sex differentiation, spermatogenesis and germ cell tumors.
| Target | Doublesex- and mab-3-related transcription factor 1 (gene DMRT1; UniProt Q9Y5R6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 39.5 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
Samples with a confirmed band (WB): rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse testis tissue, rat testis tissue, human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the N-terminal region of human DMRT1. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places doublesex- and mab-3-related transcription factor 1 in the nucleus. Tissue expression noted by UniProt (human): Testis-specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Junmei Zhang et al. (2026) Depicting the dynamic transcriptional and epigenetic landscape of testis development in pubertal Simmental cattle. Journal of Animal Science and Biotechnology. 10.1186/s40104-026-01406-x
Ming Ying et al. (2007) Nuclear import of human sexual regulator DMRT1 is mediated by importin-β. Biochimica et Biophysica ACTA-Molecular Cell Research. 10.1016/j.bbamcr.2007.03.006
Tai Shengyan et al. (2020) Alpha-Lipoic Acid Mediates Clearance of Iron Accumulation by Regulating Iron Metabolism in a Parkinson’s Disease Model Induced by 6-OHDA. Frontiers in Neuroscience. 10.3389/fnins.2020.00612
