| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1; LIG1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DNA Ligase I |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18L72 is a rabbit monoclonal antibody that recognizes DNA ligase 1 (gene LIG1) in human and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 101.7 kDa, and the supplier reports an observed band at about 140 kDa.
DNA ligase 1 (LIG1) is the main replicative DNA ligase in human cells. It joins Okazaki fragments on the lagging strand during DNA replication and seals nicks during long-patch base excision repair and other repair pathways. LIG1 is studied in DNA replication fidelity and genome stability.
| Target | DNA ligase 1 (gene LIG1; UniProt P18858, human) |
|---|---|
| Clone | 18L72 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 101.7 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Molt4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DNA Ligase I.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places DNA ligase 1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiangyang Li et al. (2019) BDE-209 induces male reproductive toxicity via cell cycle arrest and apoptosis mediated by DNA damage response signaling pathways. Environmental Pollution. 10.1016/j.envpol.2019.113097
