| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA polymerase subunit gamma-1; 3'-5' exodeoxyribonuclease; 5'-deoxyribose-phosphate lyase; Mitochondrial DNA polymerase catalytic subunit; PolG-alpha; POLG; MDP1; POLG1; POLGA |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DNA Polymerase gamma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24P93 is a rabbit monoclonal antibody that recognizes DNA polymerase subunit gamma-1 (gene POLG) in human samples and is validated for Western blot. The predicted molecular weight is 139.6 kDa, and the supplier reports an observed band at about 140 kDa.
POLG is the catalytic subunit of DNA polymerase γ, the only polymerase that copies mitochondrial DNA. Working with the helicase Twinkle and the single-strand binding protein SSBP1, it replicates the mitochondrial genome with high accuracy thanks to its proofreading activity. POLG mutations are a major cause of mitochondrial disorders.
| Target | DNA polymerase subunit gamma-1 (gene POLG; UniProt P54098, human) |
|---|---|
| Clone | 24P93 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 139.6 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human MCF-7 cells, human Jurkat cells, human Siha cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human DNA Polymerase gamma.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA polymerase subunit gamma-1 in the mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
