| Field | Specification |
|---|---|
| Target | |
| Alternative names | DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2; DNAJA1; DNAJ2; HDJ2; HSJ2; HSPF4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DNAJA1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24D64 is a rabbit monoclonal antibody that recognizes dnaJ homolog subfamily A member 1 (gene DNAJA1) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 44.9 kDa, and the supplier reports an observed band at about 45 kDa.
DNAJA1 (HDJ2) is a type I J-domain protein that works as a co-chaperone of HSP70 family proteins such as HSC70. It stimulates their ATPase activity and delivers client proteins to them for folding, and it also supports protein import into mitochondria. DNAJA1 is studied in protein quality control, apoptosis and cancer.
| Target | DnaJ homolog subfamily A member 1 (gene DNAJA1; UniProt P31689, human) |
|---|---|
| Clone | 24D64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.9 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DNAJA1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dnaJ homolog subfamily A member 1 in the membrane, cytoplasm, microsome and nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
