Anti-DNAJA4 Rabbit Monoclonal Antibody

SKU:BHA21010525
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Boster Bio
Boster Bio
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Overview
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Anti-DNAJA4 antibody (Rabbit monoclonal, clone 30D35, IgG). Validated for Western blot and immunoprecipitation. Reactivity: Human, Mouse, Rat. Recognizes dnaJ homolog subfamily A member 4. Observed band in Western blot: about 45 kDa.
Target DNAJA4
clone number 30D35
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB, IP
Options selector
Catalog no. Size Conjugation
M10527 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB,IP
      • Application details: WB 1:500-2000
        IP 1:50
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target DNAJA4
Alternative names DnaJ homolog subfamily A member 4; DNAJA4
Gene ID 55466
UniProt # Q8WW22
Host Rabbit
Clone 30D35
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human DNAJA4
Molecular weight 44.8 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Membrane; Lipid-anchor.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M10527
Main SKU BHA21010525
Rabbit Monoclonal

Product Overview

Clone 30D35 is a rabbit monoclonal antibody that recognizes dnaJ homolog subfamily A member 4 (gene DNAJA4) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 44.8 kDa, and the supplier reports an observed band at about 45 kDa.

DNAJA4 is a type I member of the DnaJ (HSP40) family of co-chaperones, which typically stimulate the ATPase activity of HSP70 proteins to support protein folding. Its specific function has not been fully characterized.

Target DnaJ homolog subfamily A member 4 (gene DNAJA4; UniProt Q8WW22, human)
Clone 30D35
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 44.8 kDa
Observed MW 45 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunoprecipitation 1:50

Immunogen

Synthetic peptide based on the sequence of human DNAJA4.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places dnaJ homolog subfamily A member 4 in the membrane.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunoprecipitation 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 44.8 kDa; observed band at about 45 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human DNAJA4.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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