| Field | Specification |
|---|---|
| Target | |
| Alternative names | DnaJ homolog subfamily C member 5; Ceroid-lipofuscinosis neuronal protein 4; Cysteine string protein; CSP; DNAJC5; CLN4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DNAJC5 recombinant protein (Position: A2-N198). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets dnaJ homolog subfamily C member 5 (gene DNAJC5) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 24 kDa.
DNAJC5 (cysteine string protein α, CSPα) is a palmitoylated J-domain co-chaperone that sits on synaptic vesicles. Working with HSC70, it helps keep the SNARE protein SNAP-25 correctly folded, supporting sustained neurotransmitter release and protecting nerve terminals from degeneration. Mutations cause adult-onset neuronal ceroid lipofuscinosis, and DNAJC5 is studied in synaptic maintenance.
| Target | DnaJ homolog subfamily C member 5 (gene DNAJC5; UniProt Q9H3Z4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 22.1 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human DNAJC5 fragment (Ala2–Asn198), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dnaJ homolog subfamily C member 5 in the cytoplasm, membrane, melanosome and cell membrane. Tissue expression noted by UniProt (human): Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
