| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA (cytosine-5)-methyltransferase 1;Dnmt1;2.1.1.37;CXXC-type zinc finger protein 9;DNA methyltransferase HsaI;DNA MTase HsaI;M.HsaI;MCMT;DNMT1;AIM, CXXC9, DNMT; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Dnmt1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AODB-4 is a rabbit monoclonal antibody that recognizes DNA (gene DNMT1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 183.2 kDa, and the supplier reports an observed band at about 200 kDa.
DNMT1 is the maintenance DNA methyltransferase that copies CpG methylation patterns onto newly synthesized DNA strands. With its partner UHRF1, it recognizes hemimethylated DNA at replication forks, allowing methylation marks to be inherited through cell division. DNMT1 is essential for development and is studied in epigenetics, genomic imprinting and cancer.
| Target | DNA (gene DNMT1; UniProt P26358, human) |
|---|---|
| Clone | AODB-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 183.2 kDa |
| Observed MW | 200 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human HepG2 cells, human 293T cells, rat NRK cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human glioblastoma tissue, rat spleen tissue, mouse lung tissue, human lung large cells cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human dnmt1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA in the nucleus and chromosome. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaowei Peng et al. (2025) DNMT3A-dependent suppression of HIF-1α alleviates myopia by reducing scleral ferroptosis and fibroblast extracellular matrix remodeling. American Journal of Pathology. 10.1016/j.ajpath.2025.11.010
Karine Assis Costa et al. (2024) Supplementation of l-arginine in pregnant gilts affects the protein abundance of DNMT1 in 35-day fetuses. Animal Reproduction Science. 10.1016/j.anireprosci.2024.107574
Bingnan He et al. (2020) β-Cypermethrin Alleviated the Inhibitory Effect of Medium from RAW 264.7 Cells on 3T3-L1 Cell Maturation into Adipocytes. Lipids. 10.1002/lipd.12234
Chengchun Shen et al. (2020) Melatonin prevents the binding of vascular endothelial growth factor to its receptor and promotes the expression of extracellular matrix‑associated genes in nucleus pulposus cells. Experimental and Therapeutic Medicine. 10.3892/etm.2020.9227
