| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA (cytosine-5)-methyltransferase 3A;Dnmt3a;2.1.1.37;DNA methyltransferase HsaIIIA;DNA MTase HsaIIIA;M.HsaIIIA;DNMT3A; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Dnmt3a |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EOI-4 is a rabbit monoclonal antibody that recognizes DNA (gene DNMT3A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 101.9 kDa, and the supplier reports an observed band at about 130 kDa.
DNMT3A is a de novo DNA methyltransferase that establishes new DNA methylation patterns during development and cell differentiation. It works with the regulatory factor DNMT3L and is guided by histone modifications. DNMT3A is one of the most frequently mutated genes in acute myeloid leukemia and clonal hematopoiesis, and it is studied in epigenetics and blood cell development.
| Target | DNA (gene DNMT3A; UniProt Q9Y6K1, human) |
|---|---|
| Clone | EOI-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 101.9 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human dnmt3a.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA in the nucleus, chromosome and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huichao Zhao et al. (2022) Folic acid intervention changes liver Foxp3 methylation and ameliorates the damage caused by Th17/Treg imbalance after long-term alcohol exposure. Food & Function. 10.1039/D1FO04267J
Zeng Peng et al. (2018) Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats. International Journal of Neuropsychopharmacology. 10.1093/ijnp/pyy090
