Anti-DNMT3B Antibody Picoband®

Overview

Anti-DNMT3B Antibody Picoband® is an antibody for DNMT3B detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: DNMT3B (Copper chaperone for superoxide dismutase); UniProt: Q9UBC3
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 96 kDa, calculated 29041 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-DNMT3B Antibody Picoband® catalog # A00319-3.

Biological background

Biological context: Delivers copper to copper zinc superoxide dismutase (SOD1).

Expression and localization notes: cellular localization: Cytoplasm ., tissue context: Ubiquitous..

Common research applications

• Western blotting (WB): Compare DNMT3B levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of DNMT3B in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify DNMT3B-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: DNA (cytosine-5-)-methyltransferase 3 beta, is an enzyme that in humans in encoded by the DNMT3B gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
• Cross reactivity: No cross-reactivity with other proteins
• Cellular localization: Cytoplasm .
• Tissue details: Ubiquitous.
• Research category: Cancer,Cardiovascular,Metabolism,Neuroscience,Neurotransmission,Plasma Membrane,Potassium Channels,Receptors / Channels,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.