| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5-hydroxymethyl-dUMP N-hydrolase; 2'-deoxynucleoside 5'-phosphate N-hydrolase 1; c-Myc-responsive protein RCL; DNPH1; C6orf108; RCL |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DNPH1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27D38 is a rabbit monoclonal antibody that recognizes 5-hydroxymethyl-dUMP N-hydrolase (gene DNPH1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 19.1 kDa, and the supplier reports an observed band at about 21 kDa.
DNPH1 is a nucleotide hydrolase that cleaves the modified nucleotide 5-hydroxymethyl-dUMP, releasing the free base. This helps remove epigenetically derived nucleotides from the nucleotide pool before they can be built into DNA. DNPH1 is studied in nucleotide pool sanitation and genome stability.
| Target | 5-hydroxymethyl-dUMP N-hydrolase (gene DNPH1; UniProt O43598, human) |
|---|---|
| Clone | 27D38 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 19.1 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human DNPH1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 5-hydroxymethyl-dUMP N-hydrolase in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
