| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dedicator of cytokinesis protein 2; DOCK2; KIAA0209 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DOCK2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25D14 is a rabbit monoclonal antibody that recognizes dedicator of cytokinesis protein 2 (gene DOCK2) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 211.9 kDa, and the supplier reports an observed band at about 212 kDa.
DOCK2 is a guanine nucleotide exchange factor for Rac that is expressed mainly in blood cells. It controls actin remodeling needed for lymphocyte migration toward chemokines and for immune cell activation. Mutations cause a combined immunodeficiency, and DOCK2 is studied in lymphocyte trafficking and immune function.
| Target | Dedicator of cytokinesis protein 2 (gene DOCK2; UniProt Q92608, human) |
|---|---|
| Clone | 25D14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 211.9 kDa |
| Observed MW | 212 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DOCK2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places dedicator of cytokinesis protein 2 in the endomembrane system and cytoplasm. Tissue expression noted by UniProt (human): Specifically expressed in hematopoietic cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
