| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein Dok-7; Downstream of tyrosine kinase 7; DOK7; C4orf25; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human DOK7, which shares 86.2% amino acid (aa) sequence identity with mouse DOK7. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein Dok-7 (gene DOK7) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 53.1 kDa, and the supplier reports an observed band at about 53 kDa.
DOK7 is a muscle adaptor protein that binds and activates the receptor tyrosine kinase MuSK. This activation is essential for clustering acetylcholine receptors and forming the neuromuscular junction. Mutations in DOK7 cause congenital myasthenic syndrome, so it is studied in neuromuscular synapse development.
| Target | Protein Dok-7 (gene DOK7; UniProt Q18PE1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 53.1 kDa |
| Observed MW | 53 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human MCF-7 cells, human RT4 cells, rat skeletal muscle tissue lysates, rat brain tissue lysates, rat heart tissue lysates, rat L6 cells lysate, mouse skeletal muscle tissue lysates, mouse brain tissue lysates, mouse heart tissue lysates, mouse C2C12 cells lysate.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human skeletal muscle, rat heart.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): RT4 cells.
Immunogen
Synthetic peptide from the middle region of human DOK7. Sequence identity with the mouse ortholog: 86.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein Dok-7 in the cell membrane and synapse. Tissue expression noted by UniProt (human): Preferentially expressed in skeletal muscle and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cheng-Dao Hua et al. (2018) Repression of Dok7 expression mediated by DNMT1 promotes glioma cells proliferation. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2018.06.156
