| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aromatic-L-amino-acid decarboxylase; AADC; DOPA decarboxylase; DDC |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DOPA Decarboxylase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17D66 is a rabbit monoclonal antibody that recognizes aromatic-L-amino-acid decarboxylase (gene DDC) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 53.9 kDa, and the supplier reports an observed band at about 53 kDa.
Aromatic L-amino acid decarboxylase (AADC, encoded by DDC) converts L-DOPA to dopamine and 5-hydroxytryptophan to serotonin. It therefore performs a key step in catecholamine and serotonin synthesis in neurons and peripheral tissues. DDC is studied in neurotransmitter metabolism, Parkinson's disease research and AADC deficiency.
| Target | Aromatic-L-amino-acid decarboxylase (gene DDC; UniProt P20711, human) |
|---|---|
| Clone | 17D66 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.9 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): 293T cells, RAW 2647 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DOPA Decarboxylase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): High expression in kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dong-Feng Chen et al. (2010) (+)-Cholesten-3-one induces differentiation of neural stem cells into dopaminergic neurons through BMP signaling. Neuroscience Research. 10.1016/j.neures.2010.07.2043
