| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium-dependent dopamine transporter; DA transporter; DAT; Solute carrier family 6 member 3; SLC6A3; DAT1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Dopamine Transporter/SLC6A3 recombinant protein (Position: M1-V620). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sodium-dependent dopamine transporter (gene SLC6A3) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 68.5 kDa, and the supplier reports an observed band at about 68 kDa.
The dopamine transporter DAT (SLC6A3) clears dopamine from the synapse back into presynaptic neurons using sodium and chloride gradients, and can also move norepinephrine. It controls the strength and duration of dopamine signaling and is the target of cocaine and amphetamine, so it is widely studied in addiction, Parkinson's disease and ADHD research.
| Target | Sodium-dependent dopamine transporter (gene SLC6A3; UniProt Q01959, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 68.5 kDa |
| Observed MW | 68 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human MCF-7 cells, human HepG2 cells, human Caco-2 cells, human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human Dopamine Transporter/SLC6A3 fragment (Met1–Val620), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sodium-dependent dopamine transporter in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Highly expressed in substantia nigra. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
S-J Hong et al. (2014) Expression of dopamine transporter in the different cerebral regions of methamphetamine-dependent rats. Human & Experimental Toxicology. 10.1177/0960327114555929
