| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc finger protein ubi-d4; Apoptosis response zinc finger protein; BRG1-associated factor 45D; BAF45D; D4, zinc and double PHD fingers family 2; Protein requiem; DPF2; REQ; UBID4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DPF2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27D77 is a rabbit monoclonal antibody that recognizes zinc finger protein ubi-d4 (gene DPF2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 44.2 kDa, and the supplier reports an observed band at about 44 kDa.
DPF2 (also called REQ or BAF45d) is a subunit of BAF (SWI/SNF) chromatin remodeling complexes. Its tandem PHD fingers recognize modified histone tails, and it has been linked to control of myeloid differentiation and the non-canonical NF-κB pathway. DPF2 mutations cause a form of Coffin-Siris syndrome, and it is studied in chromatin regulation.
| Target | Zinc finger protein ubi-d4 (gene DPF2; UniProt Q92785, human) |
|---|---|
| Clone | 27D77 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.2 kDa |
| Observed MW | 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DPF2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places zinc finger protein ubi-d4 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
