Anti-DPH5 Antibody Picoband®

Overview

Anti-DPH5 Antibody Picoband® is an antibody for DPH5 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: DPH5 (solute carrier family 2 member 6); UniProt: Q9H2P9
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 37 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-DPH5 Antibody Picoband® catalog # A11989-1.

Biological background

Biological context: Facilitative glucose transporter; binds cytochalasin B with low affinity.

Expression and localization notes: cellular localization: Cell membrane. Multi-pass membrane protein., tissue context: Highly expressed in brain, spleen and peripheral blood leukocytes..

Common research applications

• Western blotting (WB): Compare DPH5 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of DPH5 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify DPH5-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Diphthine synthase is an enzyme that in humans is encoded by the DPH5 gene. This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Multi-pass membrane protein.
• Tissue details: Highly expressed in brain, spleen and peripheral blood leukocytes.
• Research category: Cancer,Channels,Metabolism,Plasma Membrane,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.