| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dihydropyrimidine dehydrogenase [NADP; +; ]; DHPDHase; DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; DPYD |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DPYD |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25D52 is a rabbit monoclonal antibody that recognizes dihydropyrimidine dehydrogenase (gene DPYD) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 111.4 kDa, and the supplier reports an observed band at about 111 kDa.
Dihydropyrimidine dehydrogenase (DPD) is the first and rate-limiting enzyme in the breakdown of the pyrimidine bases uracil and thymine. It also inactivates the anticancer drug 5-fluorouracil, so DPYD activity and genetic variants are widely studied in pyrimidine metabolism and fluoropyrimidine pharmacology.
| Target | Dihydropyrimidine dehydrogenase (gene DPYD; UniProt Q12882, human) |
|---|---|
| Clone | 25D52 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 111.4 kDa |
| Observed MW | 111 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DPYD.
Reactivity Notes
The supplier lists reactivity with human. UniProt places dihydropyrimidine dehydrogenase in the cytoplasm. Tissue expression noted by UniProt (human): Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
