| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-C chemokine receptor type 3;C-C CKR-3;CC-CKR-3;CCR-3;CCR3;CKR3;Eosinophil eotaxin receptor;CD193;CCR3;CMKBR3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human DPYD |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-DPYD antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25D52; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-DPYD Rabbit Monoclonal Antibody catalog # M01749. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: DPYD (C-C chemokine receptor type 3).
- Antibody format: Monoclonal; clone 25D52; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
DPYD (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Reported cellular localization context: Cell membrane; Multi-pass membrane protein. Tissue expression notes (as provided): In eosinophils as well as trace amounts in neutrophils and monocytes.
Research relevance and current trends
- Research context keywords from the source record include: Antiviral Signaling,Chemokines,G Protein Signaling,Immune System Diseases,Immunology,Innate Immunity,Signal Transduction,Signaling Pathway.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate DPYD antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DPYD expression by Western blot in cell or tissue lysates, Detect DPYD in FFPE tissue sections by immunohistochemistry, Localize DPYD by immunofluorescence/immunocytochemistry in cultured cells, Quantify DPYD-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 111 kDa; calculated MW: 41044 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 111 kDa
- Cellular localization (provided): Cell membrane; Multi-pass membrane protein.
- Tissue details (provided): In eosinophils as well as trace amounts in neutrophils and monocytes.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
