| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dynamin-1-like protein; Dnm1p/Vps1p-like protein; DVLP; Dynamin family member proline-rich carboxyl-terminal domain less; Dymple; Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV; HdynIV; Dynamin-related protein 1; DNM1L; DLP1; DRP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DRP1/DNM1L recombinant protein (Position: Q618-W736). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes dynamin-1-like protein (gene DNM1L) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 81.9 kDa, and the supplier reports an observed band at about 70–75 kDa.
DRP1 (dynamin-related protein 1, encoded by DNM1L) is a large GTPase that divides mitochondria and peroxisomes. It is recruited from the cytosol by outer membrane adaptors, assembles into rings around the organelle and constricts it using GTP hydrolysis. DRP1 is studied in mitochondrial dynamics, apoptosis, mitophagy and neurodegenerative and cardiac disease.
| Target | Dynamin-1-like protein (gene DNM1L; UniProt O00429, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 81.9 kDa |
| Observed MW | 70–75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human Caco-2 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human cerebellum tissue, human tonsil tissue, human liver cancer tissue, human appendicitis tissue, human bladder cancer tissue, human gastric cancer tissue, human lung cancer tissue, human melanoma tissue, human skin cancer tissue, human testicular cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): A549 cells, SiHa cells.
Immunogen
Recombinant human DRP1/DNM1L fragment (Gln618–Trp736), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dynamin-1-like protein in the cytoplasm, Golgi apparatus, endomembrane system and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Yingying et al. (2024) Young small extracellular vesicles rejuvenate replicative senescence by remodeling Drp1 translocation-mediated mitochondrial dynamics. Journal of Nanobiotechnology. 10.1186/s12951-024-02818-5
Jing-Jing Li et al. (2023) Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow. Phytotherapy Research. 10.1002/ptr.7703
Yaqian Xu et al. (2023) Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control. Frontiers in Pharmacology. 10.3389/fphar.2023.1137609
Mengren Liu et al. (2025) GPR75 knockdown alleviates mitochondrial dysfunction in hippocampal neurons via AMPK pathway in diabetic mice. International Immunopharmacology. 10.1016/j.intimp.2025.115638
Hanyue Zheng et al. (2024) Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study. Nanomaterials. 10.3390/nano14080689
Liya Qiao et al. (2025) Promoting Drp1-Mediated Mitochondrial Division in Nickel Nanoparticles-Induced Reproductive Toxicity in GC-2 Cells. Nanomaterials. 10.3390/nano16010034
Wei Ni et al. (2022) Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model. Brain Research. 10.1016/j.brainres.2022.147978
Shaoqing Li et al. (2021) Nicorandil, an ATP-sensitive potassium channel activation, attenuates myocardial injury in rats with ischemic cardiomyopathy. Medical Molecular Morphology. 10.1007/s00795-021-00306-5
