| Field | Specification |
|---|---|
| Target | |
| Alternative names | dsRNA, Double-Stranded RNA |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Anti-dsRNA Recombinant Antibody (K2) is a mouse IgG2a kappa recombinant monoclonal antibody (clone K2) against double-stranded RNA (dsRNA). It is purified on Protein A/G from cell culture supernatant, supplied as a liquid at >95% purity as determined by SDS-PAGE, with an endotoxin level of < 10 EU/mg. The manufacturer lists it for ELISA and immunofluorescence.
The target of this antibody is a nucleic acid rather than a protein: the manufacturer gives the target as dsRNA, double-stranded RNA. Species reactivity is given as General, and the manufacturer does not break that down into individual species.
This clone carries an RRID, AB_3729644, which is the identifier to cite in a methods section.
Clone: K2 · Mouse IgG2a kappa · Protein A/G purified
Applications
| Application | Notes |
|---|---|
| ELISA | Manufacturer starting range 1:2,000–1:20,000. |
| Immunofluorescence (IF) | Listed application; no dilution, protocol or titre provided. |
The manufacturer provides no validation data or per-application protocols for these listings, so read the table as the set of applications the manufacturer supports rather than as assay-by-assay evidence. Only ELISA carries a dilution range; for immunofluorescence, establish working conditions by titration in your own system. Formats the manufacturer does not list, such as Western blot or immunohistochemistry, are uncharacterised for this clone rather than ruled out.
Specifications
| Target | dsRNA |
|---|---|
| Alternative names | Double-Stranded RNA |
| Clone | K2 |
| Host species | Mouse |
| Clonality | Monoclonal (recombinant) |
| Isotype | IgG2a, kappa |
| Species reactivity | General |
| Purification | Protein A/G purified from cell culture supernatant. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin level | < 10 EU/mg |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. Please refer to the hardcopy datasheet or the lot-specific COA supplied with the product for buffer details. |
| RRID | AB_3729644 |
| Stability and storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
Immunogen & Antigen
The manufacturer does not disclose the immunogen used to raise clone K2, and gives no epitope description beyond the target itself. There is no stated minimum duplex length, no sequence dependence and no cross-reactivity data for single-stranded RNA or DNA, so none of that can be assumed from the product data.
What the manufacturer does state is the readout: an SDS-PAGE figure for the purified antibody is provided, which speaks to the antibody preparation rather than to its performance against dsRNA in any application.
Reactivity Notes
Manufacturer-stated reactivity is General. The manufacturer does not name individual species, and does not state which sources of dsRNA the clone was tested against. Confirm detection in your own material before relying on it, and include a target-negative control alongside a known positive.
Because the antibody is a mouse IgG2a, detection requires an anti-mouse IgG secondary conjugate matched to your readout unless you label the antibody yourself. For immunofluorescence on mouse tissue or mouse cells, an anti-mouse secondary will also bind endogenous immunoglobulin, so plan the detection step accordingly.
Safety & Handling
This product is a recombinant antibody purified from cell culture supernatant. Avoid repeated freeze-thaw cycles and aliquot before long-term frozen storage. Handle as a laboratory reagent and consult the SDS before use.
For research use only.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
