| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity protein phosphatase 1;3.1.3.16;3.1.3.48;Dual specificity protein phosphatase hVH1;Mitogen-activated protein kinase phosphatase 1;MAP kinase phosphatase 1;MKP-1;Protein-tyrosine phosphatase CL100;DUSP1;CL100, MKP1, PTPN10, VH1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DUSP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IHB-4 is a rabbit monoclonal antibody that recognizes dual specificity protein phosphatase 1 (gene DUSP1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 39.3 kDa, and the supplier reports an observed band at about 45 kDa.
DUSP1 (also called MKP-1) is a dual-specificity phosphatase that removes phosphate from both threonine and tyrosine residues of MAP kinases. It is rapidly induced by stress and growth factors, provides negative feedback on MAPK signaling, and is widely studied in inflammation and stress responses.
| Target | Dual specificity protein phosphatase 1 (gene DUSP1; UniProt P28562, human) |
|---|---|
| Clone | IHB-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.3 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human DUSP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dual specificity protein phosphatase 1 in the nucleus. Tissue expression noted by UniProt (human): Expressed at high levels in the lung, liver placenta and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaoyan Wei et al. (2023) mPFC DUSP1 mediates adolescent cocaine exposure-induced higher sensitivity to drug in adulthood. EMBO Reports. 10.15252/embr.202356981
Qiang Wang et al. (2021) CMTM3 overexpression promotes cell apoptosis while DHT promotes cell proliferation in hair follicle stem cells (HFSCs). Genomics. 10.1016/j.ygeno.2020.12.029
Wei Zhang et al. (2018) USP49 inhibits ischemia–reperfusion-induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1–JNK1/2 signaling. Journal of Cellular Physiology. 10.1002/jcp.27390
