| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity protein phosphatase 6;3.1.3.16;3.1.3.48;Dual specificity protein phosphatase PYST1;Mitogen-activated protein kinase phosphatase 3;MAP kinase phosphatase 3;MKP-3;DUSP6;MKP3, PYST1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DUSP6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DBE-4 is a rabbit monoclonal antibody that recognizes dual specificity protein phosphatase 6 (gene DUSP6) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 42.3 kDa, and the supplier reports an observed band at about 42 kDa.
DUSP6 (also called MKP-3) is a cytoplasmic dual-specificity phosphatase that selectively dephosphorylates and inactivates ERK1 and ERK2. Its expression is induced by FGF and ERK signaling, forming a negative feedback loop, and it is studied in development and growth factor signaling.
| Target | Dual specificity protein phosphatase 6 (gene DUSP6; UniProt Q16828, human) |
|---|---|
| Clone | DBE-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.3 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human thyroid tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human DUSP6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dual specificity protein phosphatase 6 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in keratinocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qiang Wang et al. (2021) CMTM3 overexpression promotes cell apoptosis while DHT promotes cell proliferation in hair follicle stem cells (HFSCs). Genomics. 10.1016/j.ygeno.2020.12.029
