Description
Anti-DYKDDDDK (Flag) MagBeads are magnetic polymer beads. They are made of high quality murine monoclonal antibodies and carboxyl magnetic beads. They provide rapid magnetic responsiveness. Superparamagnetism, good dispersion, uniform particle size, very low non-specific binding and abundant binding sites.
Anti-DYKDDDDK (Flag) MagBeads can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).
Features
- Low background—little to no nonspecific binding, and no preclearing
- Highly reproducible—uniform beads ensure the most consistent results
- Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP, of low abundance proteins
- Fast and easy— no centrifugation or preclearing steps
Applications
- IP, Co-IP, pull-down, and ChIP assays
Specifications
| Clone | 1E6 |
| Isotype | Mouse IgG2b |
| MagBeads Concentration | 10 mg/mL |
| Protein binding capacity | ≥0.6 mg protein/mL |
| Storage buffer | ddH2O |
Components
| Components No. | Name | 20565ES76 | 20565ES03 | 20565ES08 |
| 20565 | Anti-DYKDDDDK (Flag) MagBeads | 500 μL | 1 mL | 5 mL |
Storage
The products should be stably stored at 2~8℃ for 1 year. Do not freeze!
The antibody is clone 1E6, isotype Mouse IgG2b. This clone is raised against the DYKDDDDK (Flag) peptide tag sequence and shows high specificity for Flag-tagged proteins with very low cross-reactivity to endogenous mammalian proteins. Protein binding capacity: ≥0.6 mg protein/mL beads.
Compatible with any protein carrying the canonical DYKDDDDK (Flag) tag — including N-terminal, C-terminal, or internal Flag tags. Also captures proteins with tandem 3×Flag tags. The antibody does not capture 1D4, VSV-G, or other unrelated epitope tags.
No pre-clearing step is needed due to very low non-specific binding. This simplifies the protocol and reduces sample loss compared to traditional bead formats. Add cell lysate directly to the beads in the recommended IP buffer.
Recommended elution options: (1) Flag peptide elution — incubate with 100–500 µg/mL DYKDDDDK peptide in IP buffer at 4°C for 15–30 min; this gives native elution preserving protein activity. (2) Low pH glycine elution (pH 2.5–3.5) — denatures proteins; neutralize immediately. (3) SDS sample buffer — for SDS-PAGE/Western blot only.
Store at 2–8°C for 1 year. Do not freeze. Storage buffer: ddH₂O. Mix by vortexing before use to ensure complete bead resuspension. The bead suspension should be uniform and brown/black in color.
The following customization and add-on services may be available for this product type through the supplier. For inquiries and pricing, contact support@biohippo.com.
Customization Options
- Bulk Quantities: Larger volumes and custom pack sizes beyond the standard catalog may be available for high-throughput core facilities and industrial customers. Contact us to inquire about bulk pricing.
- Custom Antibody Coupling (Magnetic Beads): Custom magnetic beads conjugated to your specific antibody or protein ligand may be available through Yeasen's OEM and custom services program. These services leverage the same bead platform used for catalog products.
- OEM Manufacturing: Custom bead formulations for diagnostic device development, including magnetic beads for nucleic acid isolation, IP-grade beads, and affinity capture beads, may be available at manufacturing scale.
To inquire about custom options or bulk pricing, contact support@biohippo.com.
