| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dynein light chain Tctex-type 1; Protein CW-1; T-complex testis-specific protein 1 homolog; DYNLT1; TCTEL1; TCTEX-1; TCTEX1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human DYNLT1 recombinant protein (Position: M1-A80). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets dynein light chain Tctex-type 1 (gene DYNLT1) in human, mouse and rat samples and is validated for ELISA, ICC/IF, IHC and Western blot. The predicted molecular weight is 12.5 kDa, and the supplier reports an observed band at about 16 kDa.
DYNLT1 (also called Tctex-1) is a light chain of cytoplasmic dynein 1, the main minus-end-directed microtubule motor. It helps link dynein to cargo and is studied in intracellular transport, neuronal development and ciliary function.
| Target | Dynein light chain Tctex-type 1 (gene DYNLT1; UniProt P63172, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 12.5 kDa |
| Observed MW | 16 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SiHa cells, human U251 cells, human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 13% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon adenocarcinoma tissue, human esophageal keratinizing squamous cells carcinoma tissu, human testis cancer tissue, human lung cancer tissue, mouse epididymis tissue, mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human placenta tissue, human testicular cancer tissue, rat testis tissue.
Immunogen
Recombinant human DYNLT1 fragment (Met1–Ala80), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dynein light chain Tctex-type 1 in the Golgi apparatus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
