| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor E2F1;E2F-1;PBR3;Retinoblastoma-associated protein 1;RBAP-1;Retinoblastoma-binding protein 3;RBBP-3;pRB-binding protein E2F-1;E2F1;RBBP3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human E2F1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IGD-5 is a rabbit monoclonal antibody that recognizes transcription factor E2F1 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 46.9 kDa, and the supplier reports an observed band at about 65–70 kDa.
E2F1 is the founding member of the E2F transcription factor family. Paired with a DP partner, it switches on genes needed for DNA replication and the G1-to-S transition, and its activity is held in check by the retinoblastoma protein (RB). E2F1 can also induce apoptosis and is widely studied in cell cycle control and cancer.
| Target | Transcription factor E2F1 (gene E2F1; UniProt Q01094, human) |
|---|---|
| Clone | IGD-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.9 kDa |
| Observed MW | 65–70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells lysate, human HepG2 cells, human U2OS cells, rat brain tissue, rat PC-12 cells, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human spleen tissue, human colon adenocarcinoma tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human placenta tissue, human testis cancer tissue, human thyroid papillary carcinoma tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human E2F1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor E2F1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuguo Li et al. (2022) U2 small nuclear RNA auxiliary factor 2, transcriptionally activated by the transcription factor Dp-1/E2F transcription factor 1 complex, enhances the growth and aerobic glycolysis of leiomyosarcoma cells. Bioengineered. 10.1080/21655979.2022.2061286
Guang Cao et al. (2025) Arthrocolin B Impairs Adipogenesis via Delaying Cell Cycle Progression During the Mitotic Clonal Expansion Period. International Journal of Molecular Sciences. 10.3390/ijms26041474
Wang Ping-Yu et al. (2010) Regulating A549 cells growth by ASO inhibiting miRNA expression. Molecular and Cellular Biochemistry. 10.1007/s11010-009-0380-2
Wang Xin et al. (2009) Interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-009-0611-5
