| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microtubule-associated protein RP/EB family member 3; EB1 protein family member 3; EBF3; End-binding protein 3; EB3; RP3; MAPRE3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human EB3/MAPRE3 recombinant protein (Position: D143-D268). Human MAPRE3 shares 98.4% amino acid (aa) sequence identity with both mouse and rat MAPRE3. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects microtubule-associated protein RP/EB family member 3 (gene MAPRE3) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
EB3 (MAPRE3) is an end-binding protein that follows the growing plus ends of microtubules and favors their growth and stability. It is abundant in neurons and also helps anchor non-centrosomal microtubules at the Golgi, which supports directional cell movement. Fluorescent EB3 is widely used to visualize microtubule polymerization in living cells.
| Target | Microtubule-associated protein RP/EB family member 3 (gene MAPRE3; UniProt Q9UPY8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human EB3/MAPRE3 fragment (Asp143–Asp268), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microtubule-associated protein RP/EB family member 3 in the cytoplasm. Tissue expression noted by UniProt (human): Predominantly expressed in brain and muscle.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
