| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor COE1; O/E-1; OE-1; Early B-cell factor; EBF1; COE1; EBF |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EBF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21E35 is a rabbit monoclonal antibody that recognizes transcription factor COE1 (gene EBF1) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 64.5 kDa, and the supplier reports an observed band at about 64 kDa.
Early B-cell factor 1 (EBF1) is a pioneer transcription factor that commits lymphoid progenitors to the B-cell lineage. Working with factors such as E2A and PAX5, it opens chromatin, activates B-cell genes and represses alternative fates, so it is central to studies of B-cell development and leukemia.
| Target | Transcription factor COE1 (gene EBF1; UniProt Q9UH73, human) |
|---|---|
| Clone | 21E35 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 64.5 kDa |
| Observed MW | 64 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human EBF1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places transcription factor COE1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
