| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sphingosine 1-phosphate receptor 3; S1P receptor 3; S1P3; Endothelial differentiation G protein-coupled receptor 3; Sphingosine 1-phosphate receptor Edg-3; S1P receptor Edg-3; S1PR3; C9orf108; C9orf47; EDG3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EDG3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25S21 is a rabbit monoclonal antibody that recognizes sphingosine 1-phosphate receptor 3 (gene S1PR3) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 42.3 kDa, and the supplier reports an observed band at about 42 kDa.
S1PR3 (also known as EDG3) is a G protein-coupled receptor for sphingosine 1-phosphate that can couple to several G protein classes. It influences cell survival, proliferation and migration, and it is studied in vascular biology, inflammation and fibrosis.
| Target | Sphingosine 1-phosphate receptor 3 (gene S1PR3; UniProt Q99500, human) |
|---|---|
| Clone | 25S21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.3 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human EDG3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sphingosine 1-phosphate receptor 3 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tingting Zhao et al. (2024) SPHK1/S1P/S1PR pathway promotes the progression of peritoneal fibrosis by mesothelial-mesenchymal transition. FASEB Journal. 10.1096/fj.202301323R
