| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Endothelin receptor type B; ET-B; ET-BR; Endothelin receptor non-selective type; EDNRB; ETRB |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human EDNRB, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-EDNRB Antibody Picoband® (monoclonal, 15C3) is an antibody for EDNRB detection raised in Mouse (Monoclonal, clone Clone: 15C3, Mouse IgG2b), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: EDNRB (endothelin receptor type B); UniProt: P24530
- Antibody format: Mouse, Monoclonal, clone Clone: 15C3, Mouse IgG2b
- Molecular weight: 45 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-EDNRB Antibody Picoband® (monoclonal, 15C3) catalog # M01041-1.
Biological background
Biological context: Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Expression and localization notes: cellular localization: Cell membrane. Multi-pass membrane protein., tissue context: Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells..
Common research applications
- Western blotting (WB): Compare EDNRB levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of EDNRB in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify EDNRB-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Endothelin receptor type B, also known as ETB is a protein that in humans is encoded by the EDNRB gene. The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Multi-pass membrane protein.
- Tissue details: Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
- Research category: G Protein Signaling,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
