| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Early endosome antigen 1;Endosome-associated protein p162;Zinc finger FYVE domain-containing protein 2;EEA1;ZFYVE2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human EEA1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-EEA1 Rabbit Monoclonal Antibody is an antibody targeting EEA1. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ECH-5; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 38 kDa; calculated MW: 162466 MW.
Boster Bio Anti-EEA1 Rabbit Monoclonal Antibody catalog # M02296. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: EEA1 — Early endosome antigen 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ECH-5; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 38 kDa; Calculated: 162466 MW
Biological background
Protein function (datasheet): Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Cellular localization (datasheet): Cytoplasm. Early endosome membrane; Peripheral membrane protein.
Tissue details (datasheet): Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Type Marker,Intracellular Signaling,Neuron Marker,Neuroscience,Neurotransmission,Organelle Proteins,Organelles,Protein Trafficking,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
