Anti-eEF1A1 Rabbit Monoclonal Antibody

Overview

This product is an anti-EEF1A1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20E69; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-eEF1A1 Rabbit Monoclonal Antibody catalog # M02141-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: EEF1A1 (Excitatory amino acid transporter 1).
• Antibody format: Monoclonal; clone 20E69; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

EEF1A1 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Reported cellular localization context: Membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Research relevance and current trends

• Research context keywords from the source record include: Amino Acids,Cell Type Marker,Energy Metabolism,Metabolism,Neural Stem Cells,Neuroscience,Neurotransmitter,Signal Transduction,Stem Cells,Surface Molecules,Transporters.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate EEF1A1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect EEF1A1 expression by Western blot in cell or tissue lysates, Detect EEF1A1 in FFPE tissue sections by immunohistochemistry, Localize EEF1A1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify EEF1A1-positive cells by flow cytometry in single-cell suspensions, Enrich EEF1A1 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 70 kDa; calculated MW: 59572 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 70 kDa
• Cellular localization (provided): Membrane; Multi-pass membrane protein.
• Tissue details (provided): Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.