| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pro-epidermal growth factor;EGF;Epidermal growth factor;Egf; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects pro-epidermal growth factor (gene Egf) in rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 124.1 kDa, and the supplier reports an observed band at about 6 kDa.
Epidermal growth factor (EGF) is a small growth factor cut from a large membrane precursor. By binding and activating EGFR, it stimulates proliferation and survival of epithelial cells, and in the kidney it promotes magnesium reabsorption. It is widely used in cell culture and studied in development and cancer.
| Target | Pro-epidermal growth factor (gene Egf; UniProt P07522, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 124.1 kDa |
| Observed MW | 6 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat) |
| ELISA (capture antibody) | 1–5 µg/mL (Rat) |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): rat kidney tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant rat EGF fragment (Asn974–Arg1026), expressed in E. coli. Sequence identity with the human and mouse orthologs: 69.8% and 77.4%, respectively.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places pro-epidermal growth factor in the membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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