| Field | Specification |
|---|---|
| Target | |
| Alternative names | Epidermal growth factor-like protein 6; EGF-like protein 6; MAM and EGF domains-containing gene protein; EGFL6; MAEG; PP648; UNQ281/PRO320 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human EGFL6 recombinant protein (Position: K354-D535). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes epidermal growth factor-like protein 6 (gene EGFL6) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 61.3 kDa, and the supplier reports an observed band at about 62 kDa.
EGFL6 is a secreted protein with EGF-like repeats and an integrin-binding motif. It may bind integrin α8β1 and has been linked to hair follicle development, extracellular matrix assembly and angiogenesis.
| Target | Epidermal growth factor-like protein 6 (gene EGFL6; UniProt Q8IUX8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 61.3 kDa |
| Observed MW | 62 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U-87MG cells, human A431 cells, human HL-60 cells, human K562 cells, human THP-1 cells, human HeLa cells, rat liver tissue, mouse thymus tissue, mouse lung tissue, mouse liver tissue, mouse SP20 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human EGFL6 fragment (Lys354–Asp535), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
