| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 SUMO-protein ligase EGR2; AT591; E3 SUMO-protein transferase ERG2; Early growth response protein 2; EGR-2; Zinc finger protein Krox-20; EGR2; KROX20 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human EGR2 recombinant protein (Position: M1-R257). Human EGR2 shares 90.7% amino acid (aa) sequence identity with both mouse and rat EGR2. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets E3 SUMO-protein ligase EGR2 in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
EGR2 (also called Krox-20) is a zinc finger transcription factor. It is required for hindbrain segmentation and for Schwann cell myelination of peripheral nerves, and mutations in EGR2 cause inherited peripheral neuropathies.
| Target | E3 SUMO-protein ligase EGR2 (gene EGR2; UniProt P11161, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human EGR2 fragment (Met1–Arg257), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 90.7%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 SUMO-protein ligase EGR2 in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
