Anti-EHD2 Antibody Picoband®

Overview

Anti-EHD2 Antibody Picoband® is an antibody for EHD2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: EHD2 (calbindin 2); UniProt: Q9NZN4
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 61 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-EHD2 Antibody Picoband® catalog # A04265-2.

Biological background

Biological context: Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Expression and localization notes: cellular localization: Cuticular plate. Cytosol. Nucleus. Synaptic membrane. Dendrite. Gap junction. Neuron projection. Parallel fiber to Purkinje cell synapse. Presynapse. Stereocilium. Synapse., tissue context: Brain..

Common research applications

• Western blotting (WB): Compare EHD2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of EHD2 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify EHD2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: EH-domain containing 2, also known as EHD2, is a human gene[5] belonging to the EHD protein family. This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cuticular plate. Cytosol. Nucleus. Synaptic membrane. Dendrite. Gap junction. Neuron projection. Parallel fiber to Purkinje cell synapse. Presynapse. Stereocilium. Synapse.
• Tissue details: Brain.
• Research category: Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunoglobulins,Immunology,Lymphoid,Receptors,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.