| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eukaryotic translation initiation factor 2A; eIF-2A; 65 kDa eukaryotic translation initiation factor 2A; EIF2A |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human eIF2A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18E24 is a rabbit monoclonal antibody that recognizes eukaryotic translation initiation factor 2A (gene EIF2A) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 65.0 kDa, and the supplier reports an observed band at about 65 kDa.
Eukaryotic translation initiation factor 2A (EIF2A) is a single-chain protein distinct from the eIF2 complex. It can deliver initiator methionyl-tRNA to the 40S subunit for a limited set of mRNAs and has been linked to translation from non-AUG start codons under stress.
| Target | Eukaryotic translation initiation factor 2A (gene EIF2A; UniProt Q9BY44, human) |
|---|---|
| Clone | 18E24 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 65.0 kDa |
| Observed MW | 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human Ramos cells, human Jurkat cells, human HeLa cells, human MCF-7 cells, rat pancreas tissue, mouse pancreas tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human eIF2A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Wang et al. (2018) Protective effect of miR378* on doxorubicin-induced cardiomyocyte injury via calumenin. Journal of Cellular Physiology. 10.1002/jcp.26615
