Anti-Elastin ELN Antibody (Monoclonal, BA-4)

SKU:BHA21006517
Research Validated
—
Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-ELN antibody (Mouse monoclonal, clone BA-4, Mouse IgG1). Validated for immunohistochemistry. Reactivity: Bovine, Human. Recognizes elastin. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target ELN
Clone number BA-4
Host Mouse
Reactivity Bovine,Human
Isotype Mouse IgG1
Application(s) IHC
Options selector
Catalog no. Size Conjugation
MA1038 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated
      Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
      Form: Lyophilized
      Applications: IHC
      Application details: Immunohistochemistry (Paraffin-embedded Section), 0.25-0.5μg/ml, Bovine, human
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ELN
Alternative names Elastin;Tropoelastin;ELN;
Gene ID Human(2006), Mouse(13717), Rat(25043)
UniProt # P15502
Host Mouse
Clone BA-4
Clonality
  • Monoclonal
Isotype
  • Mouse IgG1
Reactivity
  • Bovine
  • Human
Applications
  • Immunohistochemistry
Immunogen Bovine alpha-elastin.
Purification Ascites
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Cellular localization Secreted, extracellular space, extracellular matrix . Extracellular matrix of elastic fibers. .
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MA1038
Main SKU BHA21006517
Mouse Monoclonal

Product Overview

Clone BA-4 is a mouse monoclonal antibody that recognizes elastin (gene ELN) in bovine and human samples and is validated for IHC.

Elastin (ELN) is the main protein of elastic fibers, giving tissues such as large arteries, lungs and skin the ability to stretch and recoil. It is secreted as tropoelastin and cross-linked outside cells, and ELN defects cause supravalvular aortic stenosis and cutis laxa.

Target Elastin (gene ELN; UniProt P15502, human)
Clone BA-4
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG1
Reactivity Bovine, Human
Purification Ascites
Form Lyophilized
Formulation 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers
Reconstitution 100 µg/mL after adding 1 mL PBS
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 0.25–0.5 µg/mL (Bovine, Human)

Immunogen

Bovine alpha-elastin.

Reactivity Notes

The supplier lists reactivity with bovine and human. Tissue expression noted by UniProt (human): Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level).

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 0.25–0.5 µg/mL (Bovine, Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Bovine, Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on horse samples?
A.Reactivity with horse samples has not been tested by the supplier. Aligning the immunogen sequence with the horse ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Bovine alpha-elastin.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

I. Gede Mega Putra et al. (2020) Low expression of collagen type-1 in sacrouterine ligament as risk factor of stage III–IV uterine prolapse. European Journal of Obstetrics & Gynecology and Reproductive Biology. 10.1016/j.ejogrb.2020.01.027

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