| Field | Specification |
|---|---|
| Target | |
| Alternative names | ER membrane protein complex subunit 4; Cell proliferation-inducing gene 17 protein; Transmembrane protein 85; EMC4; TMEM85 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EMC4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30E32 is a rabbit monoclonal antibody that recognizes ER membrane protein complex subunit 4 (gene EMC4) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 20.1 kDa, and the supplier reports an observed band at about 18 kDa.
EMC4 is a membrane subunit of the ER membrane protein complex (EMC), which inserts newly synthesized transmembrane domains into the endoplasmic reticulum membrane without ATP. The EMC handles tail-anchored proteins and helps set the orientation of multi-pass proteins such as G protein-coupled receptors, making its subunits relevant to membrane protein biogenesis research.
| Target | ER membrane protein complex subunit 4 (gene EMC4; UniProt Q5J8M3, human) |
|---|---|
| Clone | 30E32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.1 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): 293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human EMC4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ER membrane protein complex subunit 4 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Isoform 1 is expressed in brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
