| Field | Specification |
|---|---|
| Target | |
| Alternative names | Emerin; EMD; EDMD; STA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Emerin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACGA-5 is a rabbit monoclonal antibody that recognizes emerin (gene EMD) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 29.0 kDa, and the supplier reports an observed band at about 29 kDa.
Emerin (EMD) is an inner nuclear membrane protein of the LEM-domain family that binds lamins, BAF and nuclear actin. It helps organize the nuclear envelope, links centrosomes to the nucleus and restrains nuclear β-catenin signaling. Loss of emerin causes X-linked Emery-Dreifuss muscular dystrophy, so it is widely studied in nuclear mechanics and laminopathies.
| Target | Emerin (gene EMD; UniProt P50402, human) |
|---|---|
| Clone | ACGA-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.0 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human emerin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places emerin in the nucleus inner membrane, nucleoplasmic side and nucleus outer membrane. Tissue expression noted by UniProt (human): Skeletal muscle, heart, colon, testis, ovary and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
