| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclease EXOG, mitochondrial; Endonuclease G-like 1; Endo G-like 1; EXOG; ENDOGL1; ENDOGL2; ENGL |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ENDOGL1 / ENGL |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28E53 is a rabbit monoclonal antibody that recognizes nuclease EXOG, mitochondrial in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 41.1 kDa, and the supplier reports an observed band at about 41 kDa.
EXOG is a mitochondrial endo/exonuclease related to endonuclease G. It nicks supercoiled DNA, prefers single-stranded DNA and has 5′-3′ exonuclease activity, and it is thought to process single-strand breaks during mitochondrial DNA repair.
| Target | Nuclease EXOG, mitochondrial (gene EXOG; UniProt Q9Y2C4, human) |
|---|---|
| Clone | 28E53 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.1 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ENDOGL1 / ENGL.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nuclease EXOG, mitochondrial in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
