| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ATP-binding cassette sub-family F member 1;ATP-binding cassette 50;TNF-alpha-stimulated ABC protein;ABCF1;ABC50; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Engrailed 1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-EN1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23E80; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP, Flow (as provided in the source record). Boster Bio Anti-Engrailed 1 Rabbit Monoclonal Antibody catalog # M05395. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: EN1 (ATP-binding cassette sub-family F member 1).
- Antibody format: Monoclonal; clone 23E80; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
EN1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Isoform 2 is required for efficient Cap- and IRES- mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis. . Reported cellular localization context: Isoform 2: Cytoplasm . Nucleus, nucleoplasm . Nucleus envelope . Tissue expression notes (as provided): Ubiquitous. .
Research relevance and current trends
- Research context keywords from the source record include: Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Intracellular,Neural Stem Cells,Neurology Process,Neuroscience,Nuclear,Signaling Pathways,Stem Cells,Transcription,Transcription Factors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate EN1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect EN1 expression by Western blot in cell or tissue lysates, Quantify EN1-positive cells by flow cytometry in single-cell suspensions, Enrich EN1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 55 kDa; calculated MW: 95926 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 55 kDa
- Cellular localization (provided): Isoform 2: Cytoplasm . Nucleus, nucleoplasm . Nucleus envelope .
- Tissue details (provided): Ubiquitous. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
